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Their method is based on single nucleotide polymorphism (SNP), and it is hoped that this novel approach will facilitate the detection of non-native genotypes in various species. The findings were published in Molecular Ecology Resources on August 27 (Japan time).The pond-dwelling carp (Cyprinus carpio)Foreign strains of carp from the Eurasian continent have invaded many bodies of water within Japan, and native Japanese carp are becoming rare. CREDIT: Dr. UCHII Kimiko (Faculty of Pharmacy, Osaka Ohtani University)Environmental DNA (eDNA) analysis has advanced rapidly in recent years, and is attracting increased attention. It can be used to monitor the distribution of water-dwelling organisms through extraction and analysis of the environmental DNA present in water, without capturing the organisms themselves. This is particularly effective for monitoring the invasion of non-native species which are hard to distinguish from native species by appearance.Dr. Minamoto’s research group extracted eDNA from water, and amplified and analyzed the DNA using Cycleave PCR. They developed a procedure that differentiated between and quantified the relative proportion of native and non-native genotypes, based on a single nucleotide polymorphism within a DNA sequence.Utilizing this procedure, the research group carried out empirical studies on the Japanese native common carp and the non-native strain of carp from the Eurasian continent. The Japanese native strain of common carp is endangered due to the invasion of foreign strains (these strains diverged a few million years ago). As a result of this study, the research group was able to determine the relative proportions of native and non-native strains of carp contained in the aquarium through eDNA analysis. When they used this approach in the rivers and reservoirs of western Japan to monitor the relative proportion of both carp strains, they discovered that the invasion of the foreign carp has advanced considerably.In general, foreign and native strains of same species look very similar, so it is difficult to monitor the invasion of foreign strains. Factors such as competition over resources and hybridization have had a significant negative impact on native species, and it is becoming a serious issue that threatens biodiversity. It is hoped that this method of analysis can be used to swiftly and easily determine the invasion of foreign strains in various aquatic species.Journal informationTitleA novel environmental DNA approach to quantify the cryptic invasion of non-native genotypesDOI10.1111/1755-0998.12460AuthrosUchii K, Doi H, Minamoto TJournalMolecular Ecology ResourcesRelated LinksMINAMOTO ToshifumiUCHII KimikoDOI HideyukiResearchersMinamoto ToshifumiProfessorGraduate School of Human Development and EnvironmentSDGs)">Graduate School of Human Development and EnvironmentSHAREMore from these researchersPress releases2022.02.22Agriculture and EnvironmentNew simple method for surveying amphibiansPress releases2021.05.26Agriculture and EnvironmenteDNA analysis could contribute towards more effective pest controlPress releases2019.09.02Agriculture and EnvironmentGIS and eDNA analysis system successfully used to discover new habitats of rare salamander could be utilized to find other endangered speciesPress releases2019.03.01Agriculture and EnvironmentEndangered eel located using DNA from one liter of waterMore about these topicsPress releases2017.11.17Agriculture and EnvironmentUsing eDNA to identify the breeding habitat of endangered speciesPress releases2017.01.30Agriculture and EnvironmentDNA analysis of seawater detects 80% of fish species in just one dayPress releases2016.03.07Agriculture and EnvironmentFish populations revealed through seawater analysisPress releases2021.03.04Agriculture and Environment‘Falling insect’ season length impacts river ecosystemsページの先頭へ1-1, Rokkodai-cho, Nada-ku, Kobe, 657-8501, JAPANProspective studentsCurrent studentsAlumsCorporate and societyFaculty and staffContactAccessJobsOfficial social media accountsCampus calendarPrivacy policySite policySite map© Kobe University