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神戸大学附属図書館デジタルアーカイブ 入力補助 English カテゴリ 学内刊行物 ランキング アクセスランキング ダウンロードランキング https://hdl.handle.net/20.500.14094/90006939 このアイテムのアクセス数:46件（2024-05-10 08:25 集計） 閲覧可能ファイル  ファイル フォーマット サイズ 閲覧回数 説明 90006939 (fulltext) pdf 1.35 MB 18 メタデータ ファイル出力 メタデータID 90006939 アクセス権 open access 出版タイプ Version of Record タイトル Discovery of an unrecorded population of Yamato salamander (Hynobius vandenburghi) by GIS and eDNA analysis 著者 Sakai, Yusuke ; Kusakabe, Ayane ; Tsuchida, Kota ; Tsuzuku, Yuka ; Okada, Shogo ; Kitamura, Takuto ; Tomita, Sei ; Mukai, Takahiko ; Tagami, Masataka ; Takagi, Masaki ; Yaoi, Yuichi ; Minamoto, Toshifumi 著者名 Sakai, Yusuke 著者名 Kusakabe, Ayane 著者名 Tsuchida, Kota 著者名 Tsuzuku, Yuka 著者名 Okada, Shogo 著者名 Kitamura, Takuto 著者名 Tomita, Sei 著者名 Mukai, Takahiko 著者名 Tagami, Masataka 著者名 Takagi, Masaki 著者名 Yaoi, Yuichi 著者ID A0574 研究者ID 1000050450656 KUID https://kuid-rm-web.ofc.kobe-u.ac.jp/search/detail?systemId=481adea9a93d8196520e17560c007669 著者名 Minamoto, Toshifumi 源, 利文 ミナモト, トシフミ 所属機関名 人間発達環境学研究科 収録物名 Environmental DNA 巻(号) 1(3) ページ 281-289 出版者 Wiley 刊行日 2019-09 公開日 2020-03-24 抄録 Background Biodiversity loss is a serious environmental problem, and human activities might be primarily responsible for the marked decline in animal populations globally. Amphibians, in particular, have significantly decreased in number in recent decades. One example is the endangered Yamato salamander (Hynobius vandenburghi), which is distributed in Central Japan and has a very restricted distribution in Gifu Prefecture. Aims We aimed to discover new populations of H. vandenburghi using a combination of GIS and environmental DNA (eDNA) analysis. Materials & Methods Firstly, we designed two primer sets for amplifying Hynobius species targeting mitochondrial 12S rRNA and cytochrome b genes. Next, we performed aquarium experiments to detect H. vandenburghi DNA in aquarium water. We also conducted sequential eDNA detection surveys in five known habitats in Gifu City, Japan, 17 times from January to August 2016. Following these basic eDNA studies, we used GIS to characterize the vegetation and topography of known habitats of H. vandenburghi. We collected water samples in the potential habitats identified by GIS and analyzed eDNA for the presence of H. vandenburghi using the designed primers. Finally, we conducted physical collection surveys in these potential habitats. Results We successfully developed two Hynobius-specific primer sets and detected H. vandenburghi eDNA in aquarium water. The eDNA of the target species was detected in almost all (94%?100%) samples from four habitats, whereas only 29% of samples were positive for one habitat. We identified five potential habitats by GIS analysis. The DNA of H. vandenburghi was detected in three of five potential habitats. Finally, we discovered a new population in one of the potential sites. Discussion Our approach combining GIS and eDNA enabled the detection of novel population of an endangered amphibian species. This study was conducted by high school students under the supervision of teachers with the help of university researchers, suggesting the applicability of eDNA studies as a tool of citizen science. Conclusion The combination of GIS and eDNA will allow to detect cryptic populations on which conservation efforts may be focused and to alert people to the need for conservation action. キーワード environmental DNA geographic information system Hynobius vandenburghi water カテゴリ 人間発達環境学研究科 学術雑誌論文 権利 © 2019 The Authors. Environmental DNA published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. 詳細を表示  資源タイプ journal article 言語 English (英語) eISSN 2637-4943　OPACで所蔵を検索　CiNiiで学外所蔵を検索 関連情報 DOI https://doi.org/10.1002/edn3.31 URI https://www.kobe-u.ac.jp/research_at_kobe/NEWS/news/2019_09_02_01.html ホームへ戻る お問い合わせ Kernel通信 附属図書館 Copyright © Kobe University Library All rights reserved.